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Compounds 2-4 are ester derivatives of aegelinol (1), whose absolute configuration at C-2’ was also not clear. Hydrolysis of the three esters gives the same compound (1) with a negative optical rotation. [3] decided to determinate the absolute stereochemistry by means of Mosher’s and Horeau’s (indirect) methods and assigned from their NMR data an (R) C-2’ absolute configuration for 1 and its esters 2-4.

Rigorous differentiation of enantiomers by the X-ray diffraction method is now possible through the use of the Flack parameter [7,8].

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= 6.71%) and allowed unambiguous determination of the absolute configuration of the stereogenic center of aegelinol.

The pyranocoumarin guest is included within the cylindrical cavity formed by dimeric β-cyclodextrin molecules with a head-to-head arrangement.

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